| dc.description.abstract | A total of 100 salted anchovy samples were used to investigate the prevalence of S. aureus and other coagulase positive Staphylococci (CPS) as well as to determine the methicillin (MR) and antibiotic resistance (AR) profile, the presence of Panton-Valentine leukocidine (PVL) toxin gene (lukS/F-PV), slime factor properties (SFP), and the genotypic relatedness of the isolates. Agar disc diffusion assay (ADDA) and microdilution broth susceptibility test (MDBST) were applied to compare the specificity and sensitivity of the MR detection methods. A total of 41 CPS isolates were detected at the 102 and 103 CFU/g levels in contrast to S. aureus. The 16S rRNA (genus specific) was detected in all the isolates in contrast to nuc (species-specific) and lukS/F-PV genes. A total of 16/41 isolates were found to be MR by using the three methods. Polymerase chain reaction (PCR) assay was a more sensitive and reliable method for the detection of MR isolates. The antibiotic resistance rates were 75.60, 73.17, 51.21, 31.70, 12.19, and 4.87% to penicillin, ampicillin, tetracycline, erythromycin, ciprofloxacin, and clindamycin, respectively. All the isolates were sensitive to gentamicin and vancomycin. The SFP were determined in all the isolates by using Congo Red agar, and 20 different genotypes were determined by using randomly amplified polymorphic DNA (RAPD)-PCR assay. © 2013 Taylor & Francis Group, LLC. | en_US |
| dc.contributor.department-temp | Siriken, B., Faculty of Veterinary Medicine Food Hygiene and Technology, Ondokuz Mayis University, Kurupelit Campüs, Samsun 55139, Turkey -- Yildirim, T., Department of Biology, Faculty of Science, University of Amasya, Amasya, Turkey -- Erol, I., Department of Food Hygiene and Technology, School of Veterinary Medicine, Ankara University, Ankara, Turkey -- Durupinar, B., Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey -- Ciftci, A., Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey -- Onuk, E.E., Department of Diseases and Clinical Sciences, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey | en_US |
