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Generation of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterization [Anti-salmonella enteritidis antikorlari{dotless} salgi{dotless}layan fare hibridomalari{dotless}ni{dotless}n geliştirilmesi ve ön karakterizasyonlari{dotless}]

dc.contributor.authorBüyüktanir O.
dc.contributor.authorYa?ci T.
dc.contributor.authorFindik A.
dc.contributor.authorYildirim T.
dc.contributor.authorYurdusev N.
dc.date.accessioned2019-09-01T12:50:23Z
dc.date.available2019-09-01T12:50:23Z
dc.date.issued2011
dc.identifier.issn1300-6045
dc.identifier.urihttps://hdl.handle.net/20.500.12450/695
dc.description.abstractBALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myeloma cells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to be reactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDa molecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessary to obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.en_US
dc.language.isoturen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell fusionen_US
dc.subjectHybridomaen_US
dc.subjectSalmonella enteritidisen_US
dc.subjectSpecific antibodyen_US
dc.titleGeneration of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterization [Anti-salmonella enteritidis antikorlari{dotless} salgi{dotless}layan fare hibridomalari{dotless}ni{dotless}n geliştirilmesi ve ön karakterizasyonlari{dotless}]en_US
dc.typearticleen_US
dc.relation.journalKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.identifier.startpage667en_US
dc.identifier.endpage670en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.department-tempBüyüktanir, O., Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Microbiology, TR-55139 Kurupelit, Samsun, Turkey -- Ya?ci, T., Bilkent University, Faculty of Science, Department of Molecular Biology and Genetics, TR-06100 Ankara, Turkey -- Findik, A., Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Microbiology, TR-55139 Kurupelit, Samsun, Turkey -- Yildirim, T., Amasya University, Faculty of Science, Department of Biology, TR-05100 Amasya, Turkey -- Yurdusev, N., Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Microbiology, TR-55139 Kurupelit, Samsun, Turkeyen_US


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