An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey

Abstract

In this study antibacterial, antifungal and antioxidant activities of propolis samples from the Rize province of Turkey in different solvents were investigated. A total of fifteen microorganisms belonging to Gram-positive (Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans, Streptococcus pneumoniae, Streptococcus salivarius), Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enteritidis) and a fungi (Candida albicans) were studied using a disc-diffusion and minimal inhibition concentration (MIC) methods. Ethanol extracts of propolis (EEP), acetone extracts of propolis (AEP), ethyl acetate extracts of propolis (EAEP) and methanol extracts of propolis (MEP) showed the highest antimicrobial activity against S. mutans, L. monocytogenes, M. luteus, B. licheniformis and C. albicans. While dimethyl sulfoxide extracts of propolis (DMSOEP) has the weakly activity against some test organism. The most sensitive microorganisms to propolis were E. coli, B. licheniformis, S. mutans, L. monocytogenes and B. cereus in the test microorganisms. Finally, according to the results shown by GC-MS, at least one substance was dissolved in EAEP and also by us, was found to have the highest antioxidant effect in the EAEP and AEP and the highest antimicrobial effect in the AEP

Bu çalışmada Türkiye’nin Rize ilinden toplanan propolis örneklerinin farklı solventlerde antibakteriyal antifungal ve antioksidan aktiviteleri araştırılmıştır.Gram pozitif bakteriler (Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans, Streptococcus pneumoniae, Streptococcus salivarius), Gram negatif bakteriler (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enteritidis) ve bir maya (Candida albicans) olmak üzere toplamda 15 mikroorganizma disk difüzyon ve minimum inhibisyon konsantrasyonu (MİK) yöntemi kullanılarak incelenmiştir. Propolisin etanol ekstraktı (EPE), propolisin aseton ekstraktı (APE), propolisin etil asetat ekstraktı (EAPE) ve propolisin metanol ekstraktı (MPE) S. mutans, L. monocytogenesis, M. luteus, B. licheniformis ve C. albicans’a karşı en yüksek antimikrobiyal aktivite göstermiştir. Dimetil sülfoksit Propolis Ekstraktı (DMSOPE) bazı test mikroorganizmalarına karşı zayıf aktivite göstermiştir. Propolise en duyarlı mikroorganizmalar E. coli, B. licheniformis, S. mutans, L. monocytogenes ve B. cereus olmuştur.