Antioxidant and Cytotoxic Activity Studies of Sulfur Containing Glycine Imine Derivatives MCF-7 and DLD-1 Cell Lines

Abstract

Objective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast adenocarcinoma) and DLD-1 (colorectal adenocarcinoma) cell lines. Methods: This study examined the antioxidant activities (25-200 mu M) of sulfur-containing glycine imine derivatives via the DPPH, metal chelating and reduction methods. Furthermore the cytotoxic activity of MCF-7, MCF-12A (normal breast epithelial) and DLD-1, CCD-18CO (normal colon fibroblast) were examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and RTCA (Real-time Cell Analysis) assays. Results: The antioxidant assay of the metal chelating activity showed significant results (71, 77 and 40% respectively) as compared to knowing synthetic antioxidant (trolox; 95.45, EDTA; 97.06 %). Reducing activity was found to be very low compared to the standard compounds. Compounds were shown to be moderated by DPPH (2,2-Diphenyl-1-picrylhydrazyl) activity, and the IC50 value ranged from 91 to 150. The IC50 values (100 mu M) of the MTT and RTCA analyses were similar. Conclusion: The study showed that the compounds had selective and significant antioxidant activities, and we also found that they had cytotoxic effects on MCF-7 and DLD-1 cells.