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Generation of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterization

dc.contributor.authorBuyuktanir, Ozlem
dc.contributor.authorYagci, Tamer
dc.contributor.authorFindik, Arzu
dc.contributor.authorYildirim, Tuba
dc.contributor.authorYurdusev, Nevzat
dc.date.accessioned2019-09-01T13:07:08Z
dc.date.available2019-09-01T13:07:08Z
dc.date.issued2011
dc.identifier.issn1300-6045
dc.identifier.urihttps://hdl.handle.net/20.500.12450/1600
dc.descriptionWOS: 000292686100026en_US
dc.description.abstractBALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myeloma cells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to be reactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDa molecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessary to obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.en_US
dc.description.sponsorshipState Planning Organization [DPT-2003K120900]en_US
dc.description.sponsorshipThis study was supported by State Planning Organization (Project no: DPT-2003K120900)en_US
dc.language.isoengen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSalmonella Enteritidisen_US
dc.subjectCell fusionen_US
dc.subjectHybridomaen_US
dc.subjectSpecific antibodyen_US
dc.titleGeneration of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterizationen_US
dc.typearticleen_US
dc.relation.journalKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.identifier.startpage667en_US
dc.identifier.endpage670en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.department-temp[Buyuktanir, Ozlem -- Findik, Arzu -- Yurdusev, Nevzat] Ondokuz Mayis Univ, Fac Vet Med, Dept Microbiol, TR-55139 Kurupelit, Samsun, Turkey -- [Yagci, Tamer] Bilkent Univ, Fac Sci, Dept Mol Biol & Genet, TR-06100 Ankara, Turkey -- [Yildirim, Tuba] Amasya Univ, Fac Sci, Dept Biol, TR-05100 Amasya, Turkeyen_US


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