• Türkçe
    • English
  • Türkçe 
    • Türkçe
    • English
  • Giriş
Öğe Göster 
  •   DSpace@Amasya
  • Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
  • WoS İndeksli Yayınlar Koleksiyonu
  • Öğe Göster
  •   DSpace@Amasya
  • Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
  • WoS İndeksli Yayınlar Koleksiyonu
  • Öğe Göster
JavaScript is disabled for your browser. Some features of this site may not work without it.

Investigation of Plasmid-Mediated Quinolone Resistance in Pseudomonas aeruginosa Strains Isolated from Cystic Fibrosis Patients

Erişim

info:eu-repo/semantics/closedAccess

Tarih

2011

Yazar

Coban, Ahmet Yilmaz
Cayci, Yeliz Tanriverdi
Yildirim, Tuba
Erturan, Zayre
Durupinar, Belma
Bozdogan, Bulent

Üst veri

Tüm öğe kaydını göster

Özet

Pseudomonas aeruginosa which is widely found in the environment, may lead to serious nosocomial infections. Due to its intrinsic resistance to many antibacterial agents, treatment of P.aeruginosa infections usually present difficulty. Quinolones, especially ciprofloxacin, are crutial antibiotics for the treatment of P.aeruginosa infections. However resistance developing to quinolones may become an important problem. Resistance to quinolones is often a result of chromosomal mutations and by the effect of efflux pumps. Recently plasmid-mediated quinolone resistance have been reportedin the members of Enterobacteriaceae family. The gene responsible for this resistance is called qnr. In addition to qnr genes there is also another gene called aac(6')-lb-cr responsible for plasmid-mediated quinolone resistance and aminoglycoside resistance. Limited studies which to screen P.aeruginosa strains for the presence of qnr gene region, revealed no positivity. The aim of this study was to investigate the plasmid-mediated quinolone resistance in P.aeruginosa strains isolated from cystic fibrosis patients. A total of 110 P.aeruginosa strains isolated from respiratory tract specimens from the patients were included in the study. Ciprofloxacin susceptibilities of the isolates were detected by Kirby-Bauer disk diffusion method according to CLSI guidelines. The presence of qnrA, qnrB, qnrC, qnrS and aac(6')-lb-cr genes were searched by multiplex polymerase chain reaction (PCR) with the use of specific individual primer pairs. As positive control strains, Escherichia coli J53 pMG252 (qnrA(1) positive), E.coli J53 pMG252 (qnrS(1) positive), E.coli J53 pMG258 (qnrB(1) and aac(6')-lb-cr positive), Klebsiella pneumoniae ref.15 (qnrB positive), Enterobacter cloacae ref.287 (qnrS positive), E.coli ref.20 (qnrA positive) and E.coli DH10 conjugated with pHS11 plasmid (qnrC positive) were used. Of 110 P.aeruginosa clinical isolates, 13 were found resistant to ciprofloxacin, while 7 were intermediate. However multiplex PCR yielded no positivity in terms of qnrA, qnrB, qnrC, qnrS and aac(6')-lb-cr gene regions. In conclusion, although our results indicated that none of the tested P.aeruginosa strains harboured those genes, further multicenter studies with large numbers of isolates are needed to confirm these results.

Kaynak

MIKROBIYOLOJI BULTENI

Cilt

45

Sayı

4

Bağlantı

https://hdl.handle.net/20.500.12450/1578

Koleksiyonlar

  • WoS İndeksli Yayınlar Koleksiyonu [2182]



DSpace software copyright © 2002-2015  DuraSpace
İletişim | Geri Bildirim
Theme by 
@mire NV
 

 




| Yönerge | Rehber | İletişim |

DSpace@Amasya

by OpenAIRE
Gelişmiş Arama

sherpa/romeo

Göz at

Tüm DSpaceBölümler & KoleksiyonlarTarihe GöreYazara GöreBaşlığa GöreKonuya GöreTüre GöreBölüme GöreYayıncıya GöreKategoriye GöreDile GöreErişim ŞekliBu KoleksiyonTarihe GöreYazara GöreBaşlığa GöreKonuya GöreTüre GöreBölüme GöreYayıncıya GöreKategoriye GöreDile GöreErişim Şekli

Hesabım

GirişKayıt

DSpace software copyright © 2002-2015  DuraSpace
İletişim | Geri Bildirim
Theme by 
@mire NV
 

 


|| Yönerge || Rehber || Kütüphane || Amasya Üniversitesi || OAI-PMH ||

Amasya Üniversitesi Kütüphane ve Dokümantasyon Daire Başkanlığı, Amasya, Turkey
İçerikte herhangi bir hata görürseniz, lütfen bildiriniz: openaccess@amasya.edu.tr

Creative Commons License
DSpace@Amasya by Amasya University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace@Amasya: