Basit öğe kaydını göster

dc.contributor.authorIdil, Onder
dc.contributor.authorDarcan, Cihan
dc.contributor.authorOzen, Tevfik
dc.contributor.authorOzkanca, Resit
dc.date.accessioned2019-09-01T13:06:43Z
dc.date.available2019-09-01T13:06:43Z
dc.date.issued2013
dc.identifier.issn2008-3645
dc.identifier.issn2008-4161
dc.identifier.urihttps://dx.doi.org/10.5812/jjm.4917
dc.identifier.urihttps://hdl.handle.net/20.500.12450/1528
dc.descriptionWOS: 000320698100007en_US
dc.description.abstractBackground: Light and photosensitizers affectthe survival of bacteria in natural environments. Also light and photosensitizers are used for disinfection of materials such as blood, blood products, and water. Objectives: The present study was aimed toinvestigate the effect of different wavelengths of visible light and UV-A on the synthesis of some oxidative stress enzymes of Escherichia coli (E. coli) in seawater. Materials and Methods: Seawater were filtered by using Whatmann No: 1 filter paper, followed by sterilization in the autoclave. The E. coli W3110 strain was grown at 37 degrees C, centrifuged, and transferred in seawater, then methylene blue was added to the seawater samples, with the exception of control samples. The seawater samples were incubated with white, blue, green, red, and UV-A light sources. Cell extracts were prepared by sonication, and then catalase, superoxide dismutase (SOD), glutathion peroxidase(GP), and glucose-6-phosphate dehydrogenase(G-6-PD) activities were measured. Results: It was found that in all studied wavelengths with or without Methylene Blue (MB), the level of all studied enzymes decreased remarkably when compared to dark controls. It was observed that the synthesis level of SOD, glutathione peroxidase GP, and glucose 6 phosphate dehydrogenase G-6-PD in E. coli decreased significantly in red light with respect to white, blue, and green light in seawater, to which methylene blue was added. In E. coli the decrease was 13% of G-6-PD expression, 10% of GP expression, and 17% of SOD expression in red light with MB after 16-hour incubation in seawater; however, these enzymes decreased to 45%, 84%, and 71% in white light, 33%, 47%, and 54% in blue light, 53%, 53%, and 64% in green light at the same incubation hours, respectively. Also, the enzyme acitivity in red light without MB did not show a significant difference when compared to other light sources. Conclusions: It was shown in the present study that red light among visible light sources has a crucial effect in decreasing the oxidative stress enzymes in seawater containing MB.en_US
dc.language.isoengen_US
dc.publisherAHVAZ JUNDISHAPUR UNIV MED SCIen_US
dc.relation.isversionof10.5812/jjm.4917en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRed lighten_US
dc.subjectUV-Aen_US
dc.subjectMethylene Blueen_US
dc.subjectEscherichia colien_US
dc.subjectSeawateren_US
dc.subjectPhoto-oxidationen_US
dc.titleThe Effect of UV-A and Various Visible Light Wavelengths Radiations on Expression Level of Escherichia coli Oxidative Enzymes in Seawateren_US
dc.typearticleen_US
dc.relation.journalJUNDISHAPUR JOURNAL OF MICROBIOLOGYen_US
dc.authoridOZEN, Tevfik -- 0000-0003-0133-5630; Darcan, Cihan -- 0000-0003-0205-3774en_US
dc.identifier.volume6en_US
dc.identifier.issue3en_US
dc.identifier.startpage230en_US
dc.identifier.endpage236en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.department-temp[Idil, Onder] Amasya Univ, Fac Educ, Amasya, Turkey -- [Darcan, Cihan] Bilecik Seyh Edebali Univ, Fac Arts & Sci, Dept Mol Biol & Genet, Bilecik, Turkey -- [Ozen, Tevfik] Ondokuz Mayis Univ, Fac Art & Sci, Dept Chem, Samsun, Turkey -- [Ozkanca, Resit] Meliksah Univ, Inst Sci, Kayseri, Turkeyen_US


Bu öğenin dosyaları:

DosyalarBoyutBiçimGöster

Bu öğe ile ilişkili dosya yok.

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster