Evaluation of different methods used in morphological examination of canary sperm

Abstract

By determining the species-specific structure by morphological examination of the sperm, it is possible to improve some of the disadvantages related to short and long-term storage and use in artificial insemination applications. When the morphology of the canary spermatozoon is evaluated, it is seen that it has a long indented acrosome as well as a rather long flagellum. To determine the morphological structure of canary semen, morphological examination methods used in different poultry can be applied and visually evaluated with different shapes. This study aims to provide information about the comparison and usability of various staining methods used in the morphological examination of canary semen from songbirds. In this study, semen from 12 male Gloster canaries was collected to determine morphological parameters in semen. Collected semen with different morphological evaluation methods; Fixation with 5% formaldehyde, Formalin fixation and Giemsa staining, Giemsa staining, Formalin fixation, and SpermBlue staining and SpermBlue staining were evaluated. In the results of the study, while the nucleus was more prominent in Giemsa staining compared to other staining methods used for morphological evaluation, acrosome was observed in SpermBlue and Giemsa staining. On the other hand, when the sperm fixed with 5% formaldehyde solution were evaluated, it was seen that the acrosome and nucleus were indistinguishable, while the changes in the flagellum were determined much more clearly. As a result of the study, it was reported that the morphological structure of canary semen could be evaluated with all morphological examination methods used.