Use of Extreme Low Temperatures Against Callosobruchus chinensis L. (Coleoptera: Chrysomelidae) in Storage Management

Abstract

This study was carried out to determine the effect of extreme low temperatures on different biological stages of Callosobruchus chinensis L. (Coleoptera: Chrysomelidae). Different extreme low temperatures were tested for egg, larva, pupa and adult stages in all experiments with different exposure times. For larval, pupal and adult stages, 100% mortality was obtained after 45 minutes at -16 degrees C, 30 minutes at -20 degrees C and 20 minutes at -26 degrees C. LT95 value was determined as 38.3, 39.0 and 36.8 minutes at -16 degrees C for larva, pupa and adult, respectively. LT95 value was determined as 27.4, 27.5 and 26.0 minutes at -20 degrees C for larva, pupa and adult, respectively. At the end of 120 minutes at -20 degrees C, 100% mortality was obtained in the egg stage and it was determined that the adults did not hatch from the eggs kept at -20 degrees C for 60 minutes. From these results, it is understood that extreme low temperatures are effective in all biological stages of C. chinensis that cause damage in storage.